ho 1 (OriGene)
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ho 1
Ho 1, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ho+1/Heme+Oxygenase+1+(HMOX1)+Human+qPCR+Primer+Pair/pmc13209369-269-20-24
Average 94 stars, based on 4 article reviews
Ho 1, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ho+1/Heme+Oxygenase+1+(HMOX1)+Human+qPCR+Primer+Pair/pmc13209369-269-20-24
Average 94 stars, based on 4 article reviews
ho 1 - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "Subcritical Water Extract from Grape Pomace Protects Human Bronchial Epithelium Cells by Mitigating Oxidative Stress Through Nrf2 Pathway"
Article Title: Subcritical Water Extract from Grape Pomace Protects Human Bronchial Epithelium Cells by Mitigating Oxidative Stress Through Nrf2 Pathway
Journal: Molecules
doi: 10.3390/molecules31101736
Figure Legend Snippet: SWE 160.1 treatment increased the antioxidant response. ( a ) Western blotting analysis of antioxidant factors (HO-1, SOD-2 and Nrf2) after 48 h of treatment with 75 and 100 µg GAE/mL SWE 160.1 in HBE cells. Protein levels were normalized to γ-tubulin. ( b ) qRT-PCR analysis of Nrf2, Keap1 and HO-1 mRNA levels after 24 h of treatment with 75 and 100 µg GAE/mL SWE 160.1 in HBE cells. Gene expression levels represent the relative mRNA expression compared to the untreated cells, normalized to GAPDH mRNA. ( c ) Western blotting of subcellular fractions of control and 48 h SWE 160.1 -treated cells incubated with pNrf2 (Ser40) antibody. Lamin A and GADPH antibodies marked as nuclei (N) and cytoplasmic (C) fractions, respectively. The images are representative of three different experiments. * p < 0.05, ** p < 0.01 vs. untreated control cells.
Techniques Used: Western Blot, Quantitative RT-PCR, Gene Expression, Expressing, Control, Incubation
Figure Legend Snippet: SWE 160.1 treatment overwhelmed LPS-induced HO-1-reduction. Western blotting analysis of HO-1 enzyme after 48 h of treatment with 2 µg/mL LPS alone or in combination with 100 µg GAE/mL SWE 160.1 . Protein levels were normalized to γ-tubulin. Densitometric analysis, performed using Quantity One software, (version 4.6.6), is shown in the histogram. The result is representative of two independent experiments, with values expressed as mean ± SD. ** p < 0.01 vs. untreated control cells.
Techniques Used: Western Blot, Software, Control
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Incubation:Article Title: HO-1 Limits the Efficacy of Vemurafenib/PLX4032 in BRAF V600E Mutated Melanoma Cells Adapted to Physiological Normoxia or Hypoxia Article Snippet: Protein cell lysates were denaturated in Laemmli buffer, subjected to SDS-polyacrylamide gel electrophoresis (200 Volt for 50 min) using 4–20% Mini-Protean TGX TM Gels precast (Bio-Rad). .. After blotting, PVDF membranes (GE Healthcare, Amersham Place, UK) were blocked in non-fat dry milk (5% w / v in 1X tTBS) and then incubated with antibodies against ERK, p-ERK (1:1000, rabbit polyclonal antibody, Cell Signaling Technology, Leiden, The Netherlands), Expressing:Article Title: Differentiation impairs Bach1 dependent HO-1 activation and increases sensitivity to oxidative stress in SH-SY5Y neuroblastoma cells Article Snippet: Developed films were analysed using a specific software (GelDoc; Bio-Rad). .. To study Immunofluorescence:Article Title: Differentiation impairs Bach1 dependent HO-1 activation and increases sensitivity to oxidative stress in SH-SY5Y neuroblastoma cells Article Snippet: Developed films were analysed using a specific software (GelDoc; Bio-Rad). .. To study Transfection:Article Title: Mitochondrial quality-control dysregulation in conditional HO-1 –/– mice Article Snippet: HL-1 cardiomyocytes (1 × 10 4 per treatment) were cultured in flasks coated in fibronectin (12.5 mg/l) gelatin (0.02%) containing Claycomb medium (Sigma-Aldrich) supplemented with 10% FBS (PAN Biotech GmbH), 2 mM L-glutamine (PAN Biotech GmbH), 0.1 mM norepinephrine (Sigma-Aldrich), ascorbic acid 0.3 mM (Sigma-Aldrich), 100 U/ml penicillin (Sigma-Aldrich), and 100 μg/ml streptomycin (Sigma-Aldrich) in a humid atmosphere of 5% CO 2 /95% air at 37°C ( 5 ). .. Cells were transfected in 6-well plates at 70%–80% confluence with pGFPCRS vector containing HO-1 oligonucleotide sequence for optimal suppression of Article Title: Nuclear respiratory factor-1 negatively regulates TGF-β1 and attenuates pulmonary fibrosis Article Snippet: .. Cells were transfected in 6-well plates at 70%–80% confluence with pGFPCRS vector containing HO-1 oligonucleotide sequence for optimal suppression of Article Title: Adipocyte-activated oxidative and ER stress pathways promote tumor survival in bone via upregulation of Heme Oxygenase 1 and Survivin Article Snippet: ARCaP(M) cells, an androgen-repressed metastatic prostate cancer M (‘Mesenchymal’ clone) cells, were purchased from Novicure Biotechnology (Birmingham, AL, USA). .. Both lines were stably transfected with empty vector (E) and Sequencing:Article Title: Mitochondrial quality-control dysregulation in conditional HO-1 –/– mice Article Snippet: HL-1 cardiomyocytes (1 × 10 4 per treatment) were cultured in flasks coated in fibronectin (12.5 mg/l) gelatin (0.02%) containing Claycomb medium (Sigma-Aldrich) supplemented with 10% FBS (PAN Biotech GmbH), 2 mM L-glutamine (PAN Biotech GmbH), 0.1 mM norepinephrine (Sigma-Aldrich), ascorbic acid 0.3 mM (Sigma-Aldrich), 100 U/ml penicillin (Sigma-Aldrich), and 100 μg/ml streptomycin (Sigma-Aldrich) in a humid atmosphere of 5% CO 2 /95% air at 37°C ( 5 ). .. Cells were transfected in 6-well plates at 70%–80% confluence with pGFPCRS vector containing HO-1 oligonucleotide sequence for optimal suppression of Article Title: Nuclear respiratory factor-1 negatively regulates TGF-β1 and attenuates pulmonary fibrosis Article Snippet: .. Cells were transfected in 6-well plates at 70%–80% confluence with pGFPCRS vector containing HO-1 oligonucleotide sequence for optimal suppression of other:Article Title: Lipoperoxide Nanoemulsion as Adjuvant in Cisplatin Cancer Therapy: In Vitro Study on Human Colon Adenocarcinoma DLD-1 Cells Article Snippet: Stable Transfection:Article Title: Adipocyte-activated oxidative and ER stress pathways promote tumor survival in bone via upregulation of Heme Oxygenase 1 and Survivin Article Snippet: ARCaP(M) cells, an androgen-repressed metastatic prostate cancer M (‘Mesenchymal’ clone) cells, were purchased from Novicure Biotechnology (Birmingham, AL, USA). .. Both lines were stably transfected with empty vector (E) and Plasmid Preparation:Article Title: Adipocyte-activated oxidative and ER stress pathways promote tumor survival in bone via upregulation of Heme Oxygenase 1 and Survivin Article Snippet: ARCaP(M) cells, an androgen-repressed metastatic prostate cancer M (‘Mesenchymal’ clone) cells, were purchased from Novicure Biotechnology (Birmingham, AL, USA). .. Both lines were stably transfected with empty vector (E) and Nucleic Acid Electrophoresis:Article Title: HO-1 downregulation favors BRAF V600 melanoma cell death induced by Vemurafenib/PLX4032 and increases NK recognition. Article Snippet: Heme oxygenase 1 (HO-1) plays a pivotal role in preventing cell damage.. Indeed, through the antioxidant, anti-apoptotic and anti-inflammatory properties of its metabolic products, it favors cell adaptation against different stressors.. However, HO-1 induction has also been related to the gain of resistance to therapy in different types of cancers and its involvement in cancer immune-escape has been hypothesized. |
